Figure 1 Viruses are classified based on their core genetic material and capsid design (a) Rabies virus has a singlestranded RNA (ssRNA) core and an enveloped helical capsid, whereas (b) variola virus, the causative agent of smallpox, has a doublestranded DNA (dsDNA) core and aA sucrose density gradientpurified, membranebound tobacco mosaic virus (tomato strain L) (TMVL) RNA polymerase containing endogenous RNA template was efficiently solubilized with sodium taurodeoxycholate Solubilization resulted in an increase in the synthesis of positivestrand, 64kb genomele The genome of tobacco mosaic virus (TMV) consists of a singlestranded RNA molecule of about 6,400 nucleotides in length with positive polarity, which encodes at least four polypeptides 126 and 1kDa proteins required for transcription and replication (hereafter referred to as the 126K and 1K proteins, respectively), a 30kDa (30K
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Double stranded rna is found in tobacco mosaic virus
Double stranded rna is found in tobacco mosaic virus- Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family SolanaceaeThe infection causes characteristic patterns, such as "mosaic"like mottling and discoloration on the leaves (hence the name) TMV was the first virus to beA nontransgenic approach involving the exogenous application of doublestranded RNA (dsRNA) has also been shown to induce resistance in tobacco against Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV)
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Radioactive RNA probes were prepared which specifically hybridize with sequences complementary to 5' and 3' regions of tobacco mosaic virus (TMV) RNA These probes were used in Northern hybridization to locate TMVRNA minus strands in the subcellular fractions of infected tobacco protoplasts When the protoplasts were lysed with Triton X100 Lowmolecular weight doublestranded RNA having sin glestranded tails seems to behave as a sin glestranded RNA in this extraction proce dure In the study on CCMV and BMV using CUCUMBER MOSAIC VIRUS RNAs 387 tobacco protoplasts by Bancroft et al (1975), no doublestranded RNA corre sponding to the duplicate form of RNA 4 was detected Exogenous application of doublestranded RNA (dsRNA) in the tobacco– Tobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesis
The changes in doublestranded RNA content in tobacco leaves inoculated with tomato mosaic virus (ToMV) and its avirulent mutant N14 were compared by 3Huridine incorporation and resistance to RNase It was found that the content of doublestranded RNA (dsRNA) in leaves infected with N14 was higher than in those infected with ToMVKeywords Tobacco mosaic virus, Virus small interfering RNA, C2 domain ABArelated (CAR) gene Background Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus in genus Tobamovirus It has a wide host range and its infection can cause severe damages and yield losses in many economically important crops (Scholthof et al 11) Symptoms induced by Tobacco mosaic virus (TMV) are somewhat dependent on the host plant and can include mosaic, mottling, necrosis, stunting, leaf curling, and yellowing of plant tissues The symptoms are very dependent on the age of the infected plant, the environmental conditions, the virus strain, and the genetic background of the host plant
RNAi effects were induced in P citri, as demonstrated by specific target reductions of P citri actin, chitin synthase 1 and VATPase mRNAs after injection of the corresponding specific doublestranded RNA inducers We also used recombinant Tobacco mosaic virus (TMV) to express these RNAi effectors in Nicotiana benthamiana plants Pepper mottle virus (PepMoV), a member of the genus Potyvirus which is the largest genus of plant RNA virus, infects most Capsicum sp and causes significant economic lossesPepMoV forms a flexuous rodshaped virion containing a singlestranded plus sense RNA genome of about 10 kb in length and is transmitted by aphids in a nonpersistent manner in fieldsEnzyme found in Baltimore groups VI and VII that converts singlestranded RNA into doublestranded DNA viral receptor glycoprotein used to attach a virus to host cells via molecules on the cell virion individual virus particle outside a host cell virus core contains the virus genome
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Tobacco mosaic virus protein synthesis is correlated with doublestranded RNA synthesis and not singlestranded RNA synthesis The tobacco mosaic virus, which plagues the plant but is harmless to humans, is hollowed out and filled with "small interfering RNA" molecules, or siRNA, which some scientists consider to be theDoublestranded RNA viruses are classified in two separate phyla Duplornaviricota and Pisuviricota (specifically class Duplopiviricetes), which are in the kingdom Orthornavirae and realm Riboviria The two groups do not share a common dsRNA virus ancestor Doublestranded RNA viruses evolved two separate times from positivestrand RNA viruses
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RNA duplex unwinding activity of alfalfa mosaic virus RNAdependent RNA polymerase Michael de Graaff*, Corrie J Houwing, Noemi Lukacs**, EMJ Jaspars Inslitute of Molecular Plant SCiences, Plant Virology Section, Gorlaeus Laboratories, Leiden Umverslt)', PO Box 9502, NL2300 RA Leiden, The Netherlands Received 6 July 1995 Abstract An RNAdependent RNA Each virus codes only for its coat protein, using singlestranded RNA genomes ranging from 728 nt for satellite panicum mosaic virus to 1457 nt for satellite tobacco necrosis virus Satellite tobacco mosaic virus (STMV) is the most thoroughly studied of the satellite viruses Its virion is composed of 60 identical copies of a 175 kD coatDNA is not usually found in viruses as a single polymeric strand, but as two polymers wound about each other in a double helix However viruses may contain doublestranded DNA (as in herpesviruses or poxviruses), singlestranded DNA (as in parvoviruses), doublestranded RNA (as in reoviruses) or single stranded RNA (as in picornaviruses)
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Brief Review of Literature on Tobacco Mosaic Virus 2 Review of Literature on TMVRNÀ h Review of Literature on Infective RNA 5 MATERIALS AND METHODS 9 Preparation of Virus Stocks 9 Preparation of RNA 12 The Biological Assay System 15 Analytical Ultracentrifugation Techniques 17 Equipment and Techniques for Light Scattering Experiments 31The tobacco mosaic virus (TMV) is an example of a plant virus that infects tobacco plants It is helical in shape and contains RNA It is helical in shape and contains RNA During his experiments with TMV in 12, Dmitri Ivanovsky found that some small particles of the virus could pass through the plant's filters We have examined the crucifer Tobacco mosaic virusCg (TMVCg) infection of Arabidopsis as a model system to study plantTobamovirus interaction 27,28 TMVCg is a positivesense, singlestranded RNA virus
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Tobacco Mosaic Virus Molecular Biology
Exogenous application of doublestranded RNA (dsRNA) in the tobaccoTobacco mosaic virus(TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesisIn the end, we found that strain MJM109 or the MJM109lacY mutant strain and the vector pGEMCP480 are the best choices for producing great quantities of dsRNA Resistance analyses and Northern blot showed that Tobacco mosaic virus infection could be inhibited by dsRNA, and the resistance was an RNAmediated virus resistance1 Virology 1969 Feb;37(2)276 Doublestranded tobacco mosaic virus RNA Ralph RK, Wojcik SJ PMID PubMed indexed for MEDLINE
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Viruses Microbiology
Beijerinck concluded that the cause of tobacco mosaic disease was not a filterable toxin because e the filtered sap was infectious even though microbes could not be found in it b a singlestranded RNA virus c a doublestranded DNA virus d a doublestranded RNA virusA satellite is a subviral agent that depends on the coinfection of a host cell with a helper virus for its replication Satellites can be divided into two major classes satellite viruses and satellite nucleic acids Satellite viruses, which are most commonly associated with plants, but are also found in mammals, arthropods, and bacteria, encode structural proteins to enclose their geneticHost and virus specific RNA polymerases in alfalfa mosaic virus infected tobacco Bol JF, Clerxvan Haaster CM, Weening CJ The particulate fraction of a homogenate of alfalfa mosaic virus infected tobacco was found to contain the viral replicase, and one or more hostspecific RNA
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INTRODUCTION Doublestranded tobacco mosaic virus (TMV) RNA has been detected in nucleic acid preparations from inoculated or sys temically infected leaves of virusinfected tobacco plants This material appears to be the active intermediate in TMV RNA syn thesis The site of synthesis of TMV RNA is at present disputedRabies virus is a () strand RNA virus that transcribes mRNAs from its genome (Group V) HIV1 is a singlestranded RNA retrovirus that uses reverse transcriptase to create a doublestranded DNA copy of its genome which is integrated into the host human's genome prior toDoublestranded RNA It has been found within viral capsid in the reoviruses of animals and in the wound tumour virus and rice dwarf viruses of plants Viroid Small viruses without capsid Singlestranded RNA It is found in most of the RNA viruses eg tobacco mosaic virus, influenza virus, poliomyelitis, bacteriophage MS2, Avian leukemia virus
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Mutation Studies With Tobacco Mosaic Virus Technical Progress Report December 1 1969 November 30 1970 Unt Digital Library
MEDIUM View Answer Sometimes when a virus attacks a bacterium, neither the virusViral Morphology Viruses are noncellular, meaning they are biological entities that do not have a cellular structureThey therefore lack most of the components of cells, such as organelles, ribosomes, and the plasma membrane A virion consists of a nucleic acid core, an outer protein coating or capsid, and sometimes an outer envelope made of protein and phospholipidA double stranded RNA molecule C A single stranded DNA molecule D A double stranded DNA molecule EASY The filterable property of tobacco mosaic virus (TMV) was shown by EASY View Answer Which of the following sequence is found in Rous sarcoma virus?
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However, the enzyme does not requireTobacco Mosaic Virus is a ribovirus and contains single stranded RNA It was proved by the experiments of Frankel Conart that RNA is the genetic material in this virus It does not contain any DNA and is composed of 6% RNA surrounded by a hollow cylinder of portion subunits Double stranded RNA is found in Reovirus and Tumor virusVirusspecific RNA isolated from TMVinfected plants contains, in addition to genomic and subgenomiclength positivestrand RNA, genomiclength doublestranded RNA, designated replicativeform (RF) RNA and a heterogeneous family of RNAs, which are partly doublestranded and partly singlestranded, designated replica
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Small Rna Derived From Tobacco Mosaic Virus Targets A Host C2 Domain Abscisic Acid Related Car 7 Like Protein Gene Phytopathology Research Full Text
Figure 215 Viruses are classified based on their core genetic material and capsid design (a) Rabies virus has a singlestranded RNA (ssRNA) core and an enveloped helical capsid, whereas (b) variola virus, the causative agent of smallpox, has a doublestranded DNA (dsDNA) core and a complex capsid The changes in doublestranded RNA content in tobacco leaves inoculated with tomato mosaic virus (ToMV) and its avirulent mutant N 14 were compared by 3 Huridine incorporation and resistance to RNase It was found that the content of doublestranded RNA (dsRNA) in leaves infected with N 14 was higher than in those infected with ToMV At the secondTobacco Mosaic Virus Testable Question Experimental Method Mendel Scientific Method TERMS IN THIS SET (11) In the early days of germ theory, contagious diseases were thought to be caused by fungi or bacteria In the 10s, Dmitri Ivanovski filtered extracts from diseased tobacco plants and discovered that the disease could be transmitted to
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Tobacco Mosaic Virus Wikipedia
Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family Solanaceae The infection causes characteristic patterns, such as " mosaic "like mottling and discoloration on the leaves (hence the name)The RNAdependent RNA polymerase (replicase) mediating the replication of tobacco mosaic virus (TMV) has been investigated in a number of laboratories over a period of years Cellfree enzyme preparations have been prepared which can continue the synthesis of nascent complementary RNA, initiated in vivo;
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